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African Journal of Biotechnology

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Type I collagen from bullfrog (Rana catesbeiana) fallopian tube

T Wang, S Lin, Y Shen, S Liu, T Wang-McCall, M Chin, T Lin, C Yang, W Wu, C Yang

Abstract


Pepsin-soluble collagen (PSC) was extracted from the fallopian tube of bullfrog (Rana catesbeiana) with a yield of 16.4%, on a dry weight basis. Sodium dodecyl sulphate polyacylamide-gel electrophoresis (SDS-PAGE) showed that the PSC contained two alpha components (α1 and α2) and was classified as type I collagen. PSC was found to contain 19.5% of amino acids. PSC exhibited a maximum absorbance at 230 nm, but little absorbance near to 280 nm. The denaturation temperature for PSC was determined to be 29.6°C. These results suggest that bullfrog fallopian tube has potential as a supplement to the sources of vertebrate collagens.

Key words: Bullfrog (Rana catesbeiana), pepsin-soluble collagen, type I collagen.




http://dx.doi.org/10.5897/AJB10.924
AJOL African Journals Online