Type I collagen from bullfrog (Rana catesbeiana) fallopian tube

  • T Wang
  • S Lin
  • Y Shen
  • S Liu
  • T Wang-McCall
  • M Chin
  • T Lin
  • C Yang
  • W Wu
  • C Yang
Keywords: Bullfrog (Rana catesbeiana), pepsin-soluble collagen, type I collagen.

Abstract

Pepsin-soluble collagen (PSC) was extracted from the fallopian tube of bullfrog (Rana catesbeiana) with a yield of 16.4%, on a dry weight basis. Sodium dodecyl sulphate polyacylamide-gel electrophoresis (SDS-PAGE) showed that the PSC contained two alpha components (α1 and α2) and was classified as type I collagen. PSC was found to contain 19.5% of amino acids. PSC exhibited a maximum absorbance at 230 nm, but little absorbance near to 280 nm. The denaturation temperature for PSC was determined to be 29.6°C. These results suggest that bullfrog fallopian tube has potential as a supplement to the sources of vertebrate collagens.

Key words: Bullfrog (Rana catesbeiana), pepsin-soluble collagen, type I collagen.

Published
2013-10-17
Section
Articles

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eISSN: 1684-5315