Micropropagation of rose cultivar ‘Pareo’

Abstract

A protocol was developed for micropropagation of rose cv. ‘Pareo’. Nodal segments were surface sterilized with 0.1% solution of mercuric chloride for 10 min and these disinfected explants were inoculated aseptically on culture medium. The, axillary shoots were regenerated from nodal explants on agar-gelled Murashige and Skoog medium supplemented with 1.5 mg/l BAP. In vitro rooting was obtained when shoot clusters were cultured on half strength Murashige and Skoog medium supplemented with 1.0 mg/l IBA. The rooted plantlets were acclimatized successfully in the field.

Key words: Micropropagation, nodal segments, rose.