Effect of the Chinese herb Mesima Reishi UE-1 on invasion of human ovarian cancer cells in vitro

  • J He
  • X He
  • HY Zhang
  • XF Yang
  • J He
  • LH Zhang
  • YL Li
Keywords: Mesima Reishi UE-1, polysaccharides, human ovarian cancer, tumor invasion, matrix metalloproteinase

Abstract

To study the effects of Mesima Reishi UE-1 on the metastatic phenotype of the human ovarian cancer cell line HO8910. HO8910 cells were pretreated with different concentration of Mesima Reishi UE-1 in vitro. Using cell proliferation assay, spreading and adhesion assay, cell migration and invasion assay, zymography, immunocytochemical staining and RT-PCR, we examined the effects of Mesima Reishi UE- 1 on cell proliferation, motility, adhesion and expression of MMP-2, 9 in vitro. Mesima Reishi UE-1 directly inhibited HO8910 cell proliferation in vitro. And pretreated with different concentrations of Mesima Reishi UE-1, HO8910 cells motility, adhesion and invasion were inhibited significantly. The activity of MMP-9 was inhibited in a dose-dependent manner. The protein and mRNA expression of MMP-2, 9 were also inhibited. Mesima Reishi UE-1 suppresses invasion of human ovarian cancer cells in vitro.

Key words: Mesima Reishi UE-1, polysaccharides, human ovarian cancer, tumor invasion, matrix metalloproteinase.

Published
2013-10-24
Section
Articles

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eISSN: 1684-5315