African Journal of Biotechnology

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High-frequency shoot regeneration of nodal explants from Tetrastigma hemsleyanum Diels et Gilg: A valuable medicinal plant

W Jiang, Y Fu, X Zhou, C Fu


This paper describes the shoot regeneration of nodal segments from a medicinal plant, Tetrastigma hemsleyanum Diels et Gilg (Vitaceae). The highest number of shoots (7.27 shoots per explant) was observed in MS medium supplemented with 4 mg/l BA after six weeks of inoculation. 2 mg/l BA in combination with 0.1 mg/l NAA not only induced shoot proliferation but also increased shoot length. Well-developed shoots were rooted on half strength MS medium supplemented with 2 mg/l IBA with 100% rooting and 85% of the regenerated plantlets survived before been transferred to field conditions.

Key words: Medicinal plant, nodal explants, shoot regeneration, Tetrastigma hemsleyanum.

AJOL African Journals Online