In vitro anti-trypanosomal activities of crude extracts, β-sitosterol and α-sulphur from Buchholzia coriacea seed

  • NE Nweze
  • BM Anene
  • IU Asuzu
Keywords: Trypanosoma brucei brucei, Buchholzia coriacea, bioassay-guided fractionation


The seeds of Buchholzia coriacea Engler are traditionally used as a febrifuge in Southeast Nigeria. The bioassay-guided fractionation of the crude extracts was carried out using in vitro cultures of Trypanosoma brucei brucei S427. On soxhlet extraction, methanol gave the highest yield (5.44 %) followed by hexane (3.34%) and ethyl acetate (0.66%). The ethyl acetate extract showed the highest anti-trypanosomal activity followed by the hexane and methanol extracts. Beta-sitosterol and α–sulphur were isolated from B. coriacea seed extracts by preparative chromatography and identified by nuclear magnetic resonance and x-ray crystallography, respectively. Beta-sitosterol and α–sulphur showed modest anti-trypanosomal activities against T. b. brucei S427 in vitro with minimum inhibitory concentration (MIC) values of 12.5 and 25 μg/ml respectively.

Key words: Trypanosoma brucei brucei, Buchholzia coriacea, bioassay-guided fractionation.


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eISSN: 1684-5315