PROMOTING ACCESS TO AFRICAN RESEARCH

African Journal of Biotechnology

The AJOL site is currently undergoing a major upgrade, and there will temporarily be some restrictions to the available functionality.
-- Users will not be able to register or log in during this period.
-- Full text (PDF) downloads of Open Access journal articles will be available as always.
-- Full text (PDF) downloads of subscription based journal articles will NOT be available
We apologise for any inconvenience caused. Please check back soon, as we will revert to usual policy as soon as possible.





Metagenomic analysis of bacterial diversity of Siloam hot water spring, Limpopo, South Africa

M Tekere, A Lötter, J Olivier, N Jonker, S Venter

Abstract


The bacterial diversity of Siloam hot water spring was determined using 454 pyrosequencing of two 16S rRNA variable regions V1-3 and V4-7. Analysis of the community DNA revealed that the phyla Proteobacteria, Cyanobacteria, Bacteriodetes, Planctomycetes, Firmicutes, Chloroflexi and Verrucomicrobia were the most abundant. The bacterial diversity detectable and classifiable was greater when the V4-7 variable region was used compared to the V1-3 region. The most abundant bacteria genera detected with region V1-3 were; Stenotrophomonas (23.3%), Aquaspirillum (5.11%), Zavarzinella (2.73%), Haliscomenobacteria (1.25%), Rheinheimera (1.14%) and Tepidomonas (1.14%). All the other detectable genera were below 0.6%. Genera detected with region V4-7 from most abundant were; Stenotrophomonas (17.96%), Zavarzinella (5.81%), Aquaspirillum (4.75%), Rheinheimera (3.52%), GPI (1.41%), Gemmata (1.41%) and Syntrophobacter (1.06%). All the other genera detected were below 0.7%. Siloam is one of the hottest thermal springs in South Africa (63°C), the water has a pH of 9.5 and is relatively high in fluoride and bromide; it is possible that the physicochemical properties could have some influence on the diversity of bacteria. This article reports on the first phylogenetic analysis of a South African thermal spring bacterial community.

Key words: Thermophilic, hot springs, biodiversity, 454 sequencing, South Africa.




http://dx.doi.org/10.5897/AJB11.899
AJOL African Journals Online