Evaluation of the anti-oxidant and anti-angiogenic effects of Sphenocentrum Jollyanumpierre

Abstract

The methanol extracts of Sphenocentrum Jollyanum organs were assessed for their anti-oxidant and anti-angiogenic activities using DPPH and CAM assays respectively. The results indicated the stem bark as the most active organ with an IC50 of 1.80 ± 0.25 and 1.00 ± 0.20 score (at 500 ìg/pellet) on DDPH and anti-angiogenesis assays respectively. Further fractionation of the stem bark revealed the chloroform fraction to have the highest IC50 1.54 ± 0.15 and the most important score on anti-angiogenesis assay with 1.3 ± 0.10 at 250 ìg/pellet when serially diluted between 250 and 36.2 ìg/pellet. Moreover, the effects were found to be dose-dependent. These results bring to the fore the need for further studies towards confirming the anti-oxidant and antiangiogenic potentials of the plant as well as identifying and characterizing the active principles for drug development