African Journal of Infectious Diseases

Log in or Register to get access to full text downloads.

Remember me or Register

Comparison of anti bacterial efficacy of photodynamic therapy and doxycycline on aggregatibacter actinomycetemcomitans

Ernie Maduratna Setiawatie, Vina Puji Lestari, Suryani Dyah Astuti


Background: Aggregatibacter actinomycetemcomitans (A. actinomycetemcomitans) is an anaerobic bacterium has been frequently associated with aggressive periodontitis. Photodynamic therapy (PDT) is a medical treatment to prevent infection progression that utilizes light to activate a photosensitizing agent. Doxycycline is an antibacterial having pho-tosensitivity. This study aimed to evaluate potential doxycycline as an antibacterial and photosensitizer combine PDT against A. actinomycetemcomitans bacteria.

Material and methods: Samples were distributed to 4 groups as follow: (1) Groups A treated with a diode laser, (2) Group B treated with doxycycline 0,1% and laser, (3) Group C treated only with Doxycycline 0.1%, and (4) Group D no exposure doxycycline/laser. Data were analyzed by one-way ANOVA and Tukey's HSD test at 5% significance level.

Results: In this study, doxycycline 0.1% has the effect of reducing the bacterial viability of (59.60±3.26%). Whereas laser exposure 120 s combined with doxycycline produce the effect of bacterial viability reduction (88.50±2.83%) is not significantly different from the effect of laser 120 s exposure (88.79±2.60%). In clinical treatment, the application of laser exposure is generally at the duration of the exposure time of 30 s. The results of this study indicate that the du-ration of laser exposure 30 s shows a reduction in bacterial viability (44.91±1.69%) equal to the laser and with a com-bination of doxycycline and laser exposure (70.70±2.43%). So at low doses of laser exposure, doxycycline 0.1% com-bined with the laser results in the greatest reduction in bacterial viability, significantly different from laser exposure alone.

Conclusion: at low doses of laser exposure (30s with energy 3.68 J/cm2), doxycycline 0.1% combined with the laser results in the greatest reduction in bacterial viability, significantly different with laser exposure alone.

Keywords: Photodynamic therapy, doxycycline, Aggregatibacter actinomycetemcomitans
AJOL African Journals Online