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African Journal of Infectious Diseases

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Comparison of anti bacterial efficacy of photodynamic therapy and doxycycline on aggregatibacter actinomycetemcomitans

Ernie Maduratna Setiawatie, Vina Puji Lestari, Suryani Dyah Astuti

Abstract


Background: Aggregatibacter actinomycetemcomitans (A. actinomycetemcomitans) is an anaerobic bacterium has been frequently associated with aggressive periodontitis. Photodynamic therapy (PDT) is a medical treatment to prevent infection progression that utilizes light to activate a photosensitizing agent. Doxycycline is an antibacterial having pho-tosensitivity. This study aimed to evaluate potential doxycycline as an antibacterial and photosensitizer combine PDT against A. actinomycetemcomitans bacteria.

Material and methods: Samples were distributed to 4 groups as follow: (1) Groups A treated with a diode laser, (2) Group B treated with doxycycline 0,1% and laser, (3) Group C treated only with Doxycycline 0.1%, and (4) Group D no exposure doxycycline/laser. Data were analyzed by one-way ANOVA and Tukey's HSD test at 5% significance level.

Results: In this study, doxycycline 0.1% has the effect of reducing the bacterial viability of (59.60±3.26%). Whereas laser exposure 120 s combined with doxycycline produce the effect of bacterial viability reduction (88.50±2.83%) is not significantly different from the effect of laser 120 s exposure (88.79±2.60%). In clinical treatment, the application of laser exposure is generally at the duration of the exposure time of 30 s. The results of this study indicate that the du-ration of laser exposure 30 s shows a reduction in bacterial viability (44.91±1.69%) equal to the laser and with a com-bination of doxycycline and laser exposure (70.70±2.43%). So at low doses of laser exposure, doxycycline 0.1% com-bined with the laser results in the greatest reduction in bacterial viability, significantly different from laser exposure alone.

Conclusion: at low doses of laser exposure (30s with energy 3.68 J/cm2), doxycycline 0.1% combined with the laser results in the greatest reduction in bacterial viability, significantly different with laser exposure alone.

Keywords: Photodynamic therapy, doxycycline, Aggregatibacter actinomycetemcomitans




http://dx.doi.org/10.21010/ajid.v12i1S.14
AJOL African Journals Online