PROMOTING ACCESS TO AFRICAN RESEARCH

African Journal of Infectious Diseases

Log in or Register to get access to full text downloads.

Remember me or Register



Effect of varying incubation periods on cytotoxicity and virucidal activities of Justicia gendarussa Burm.f. leaf extract on HIV-infected MOLT-4 cells

Prihartini Widiyanti, Bambang Prajogo, Agustinus Widodo

Abstract


Backgrounds: Justicia gendarussa Burm.f. has an anti-HIV activity. This study was conducted to evaluate the effects of incubation periods on the cytotoxicity and virucidal activities of the J. gendarussa leaves extract on MOLT-4 cells.

Materials and Methods: The cytotoxicity assay was evaluated by using the WST-1 test with incubation periods of 3 days and 5 days. The virucidal activity test was determined by measuring the inhibitory activities on the syncytium formation.

Results: The cytotoxicity assay showed the value of CC50 on MOLT-4 cell culture with the test material of 70% ethanol extract of J. gendarussa leaves as much as 3928.620 μg /mL and 3176.581 μg /mL (incubation day 3 and day 5, respectively); fractionated-70% ethanol extract = 81782.428 μg /mL and 12175.870 μg/mL; and water extract = 16372.689 μg/mL and 2946.117 μg/mL. The test results of the virucidal activities (inhibit ≥ 90% the formation of syncytium) of 70% ethanol extract of J. gendarussa leaves is at a concentration 250 μg/mL, 500 μg/mL and 1000 μg/mL (3-day incubation) and 250 μg/mL (5-day incubation); and fractionated-70% ethanol extract at a concentration 250 μg /mL, 500 μg/mL and 1000 μg/mL (3-day incubation) and 1000 μg/mL (5-day incubation).

Conclusion: 70% ethanol extract, fractionated-70% ethanol extract, and water extract of J. gendarussa leaves were relatively nontoxic toward MOLT-4 cells, and fractionated-70% ethanol extract had better potentials in virucidal activities.

Keywords: Justicia gendarussa Burm.f., MOLT-4 cells, cytotoxicity, virucidal, incubation periods




AJOL African Journals Online