A microsatellite panel for triploid verification in the abalone Haliotis midae

  • R Slabbert
  • N Prins
  • D Brink

Abstract

A method for ploidy verification of triploid and diploid Haliotis midae was developed using molecular microsatellite markers. In all, 30 microsatellite loci were tested in control populations. A final micro satellite multiplex consisting of seven markers were optimised and a complete protocol is reported. This protocol was successfully applied in identifying the ploidy levels of 42 ploidy-unknown abalone, showing the utility of microsatellite markers as tools for verifying the ploidy of individual abalone. The protocol can be applied in larger animals for which the isolation of nuclei and metaphase chromosomes from tissues is difficult.

Keywords: abalone, Haliotis midae, microsatellites, triploidy

Author Biographies

R Slabbert
Aquaculture Division, Department of Genetics, Stellenbosch University, Private Bag X1, Matieland 7602, South Africa
N Prins
Aquaculture Division, Department of Genetics, Stellenbosch University, Private Bag X1, Matieland 7602, South Africa
D Brink
Aquaculture Division, Department of Genetics, Stellenbosch University, Private Bag X1, Matieland 7602, South Africa
Section
Articles

Journal Identifiers


eISSN: 1814-2338
print ISSN: 1814-232X