PROMOTING ACCESS TO AFRICAN RESEARCH

African Journal of Marine Science

Log in or Register to get access to full text downloads.

Remember me or Register



DOWNLOAD FULL TEXT Open Access  DOWNLOAD FULL TEXT Subscription or Fee Access

A microsatellite panel for triploid verification in the abalone Haliotis midae

R Slabbert, N Prins, D Brink

Abstract


A method for ploidy verification of triploid and diploid Haliotis midae was developed using molecular microsatellite markers. In all, 30 microsatellite loci were tested in control populations. A final micro satellite multiplex consisting of seven markers were optimised and a complete protocol is reported. This protocol was successfully applied in identifying the ploidy levels of 42 ploidy-unknown abalone, showing the utility of microsatellite markers as tools for verifying the ploidy of individual abalone. The protocol can be applied in larger animals for which the isolation of nuclei and metaphase chromosomes from tissues is difficult.

Keywords: abalone, Haliotis midae, microsatellites, triploidy



http://dx.doi.org/10.2989/1814232X.2010.501570
AJOL African Journals Online