RAPD and SSR based genetic diversity analysis of elite-2 set of synthetic hexaploid wheats

  • K Ahmad
Keywords: Elite-II, molecular markers, RAPD, SSR, synthetic hexaploid wheat

Abstract

Background: Synthetic hexaploid wheats are artificially reconstituted  hexaploid wheats that possess high genetic variation which could be utilized for the development of new improved wheat varieties. One such group of synthetic wheats is called the Elite-II set of synthetic wheats that are derived from crossing durum wheat with different Aegilops tauschii wheats.
Materials and Methods: In the current study genetic diversity was  investigated among 18 Elite-II synthetic hexaploid wheat lines at DNA level. Two types of molecular markers i.e. RAPD and SSR were used for this purpose.
Results: Both types of markers proved useful in estimating the overall genetic diversity among these lines. Based on RAPD data range of genetic
distances in these lines was from 0 to 100 percent. Seven D- genome  specific SSRs were also used to get further estimation of the genetic diversity contributed by Aegilops tauschii parent. On the basis of results obtained it is inferred that the Aegilops tauschi accessions used in the
production of these synthetic lines were genetically different and they  contributed to the enhancement of genetic variation in the synthetic lines.
These results could be helpful for future genome mapping programs.
Conclusion: The overall extensive genitive diversity indicates that these lines are good candidates for development of improved wheat varieties by crossing with cultivated wheat varieties.

Key words: Elite-II, molecular markers, RAPD, SSR, synthetic hexaploid wheat

Published
2014-08-11
Section
Articles

Journal Identifiers


eISSN: 0189-6016