Effects of Ciji Hua’Ai Baosheng formula on apoptosis correlation factors of tumor chemotherapy model mouse with H22 hepatomacarcinoma cells
Background: Side effects of chemotherapy are major issues for cancer patients and there are few methods to release these. However, traditional Chinese medicine is one of the options for these patients. This study is to evaluate one traditional Chinese empirical formula – the Ciji Hua’ai Baosheng Formula (CHBF) on apoptosis related factors in a transplanted tumor chemotherapy model.
Materials and Methods: H22 hepatoma cells were injected into peritoneal cavity and Cytoxan (CTX) (200mg/kg) was used to treat these carcinoma cells. Mice were divided into control, CTX control, and CTX plus three different concentrations of CHBF groups. H22 hepatoma cells proliferation, serum levels of apoptosis related factors, and bone marrow cyclin D1 expression was evaluated.
Results: After treatment of CHBF, H22 hepatoma cell proliferation was lower than that in CTX group. The pro-apoptosis related proteins Bax and Caspase-3 were elevated while anti-apoptosis related protein Bcl-2 was reduced. Moreover, serum epidermal growth factor receptor level and bone marrow cyclin D1 expression were significantly reduced in CHBF treated groups.
Conclusion: The Ciji Hua’ai Baosheng Formulae could modulate apoptotic and proliferative factors in a model of tumor with chemotherapy, which may be the mechanisms why it can release chemotherapy related side effect in patients with cancer.
Key words: Ciji Hua’ai Baosheng Formula (CHBF); tumor chemotherapy model; Bax; Bcl-2; Caspase-3; epidermal growth factor receptor (EGFR); CyclinD1; H22 hepatoma carcinoma cell
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