Serum protein profile of Malaria patients through SDS-PAGE method
Background: Malaria is a crucial disease that causes huge mortality and morbidity, along with economic losses in term of purchasing medicines in underdeveloped countries such as Pakistan.
Materials and Methods: A total of 150 human blood samples were collected from the Districts of Karak and Kohat Khyber Pakhtunkhwa, in Pakistan, and were examined through microscopy and then all positive samples were analyzed by SDS-PAGE method for the detection of antibodies against plasmodium.
Result: The overall prevalence of malaria was found to be 18% (27/150). The prevalence of plasmodium species was “21.3% (16/75) and 14.6% (11/75) in the Districts of Karak and Kohat respectively. The prevalence of plasmodium species i.e. P. vivax and P. falciparum, in the Districts of Karak and Kohat, was found to be 14.6% (22/150) and 3.33% (5/150) respectively. Among these, P. vivax 10.6% (8/75) and P.falciparum 4% (3/75) were noted in the District of Kohat; while P. vivax 18.6% (14/75) and P. falciparum 2.6% (2/75) were found in the District of Karak Khyber Pakhtunkhwa, Pakistan respectively”. The serum protein of malaria patients were 57KDA and 78KDA bands, which were determined by Sodium Dodecyl Sulfate Poly-Acrylamide Gel Electrophoresis (SDS-PAGE) technique.
Conclusion: The SDS page method is the most accurate diagnostic assay and was recognized as a most reliable method in the diagnosis of antibodies produced against Plasmodium spps. The proteins bands of Serum protein of malaria patients may be undertaken for study to develop possible future vaccine.
Key words: Malaria, Serum protein, SDS-PAGE, microscopy and Plasmodium species