Article Sidebar
Published:
Jun 5, 2018Keywords:
Article Details
Copyright: Creative Commons Attribution CC.
This license lets others distribute, remix, tweak, and build upon your work, even commercially, as long as they credit you for the original creation. This is the most accommodating of licenses offered. Recommended for maximum dissemination and use of licensed materials. View License Deed | View Legal Code Authors can also self-archive their manuscripts immediately and enable public access from their institution's repository. This is the version that has been accepted for publication and which typically includes author-incorporated changes suggested during submission, peer review and in editor-author communications.
Main Article Content
<b>Retracted:</b> <i>Ananas sativa</i> increases longevity through oxidative stress resistance in <i>Drosophila melanogaster</i>
Abstract
This article has been retracted by the Editor.
Background: Ageing is the major risk factor for most non-communicable diseases. It occurs as a result of free radical damage to macromolecules in the cell, coupled with environmental stress assaults. In the quest to identify new anti-ageing bioactive compounds from natural products, and despite the therapeutic values of Ananas sativa, its anti-ageing activity has not been fully elucidated. Therefore, the aim of this study was to screen the crude, methanol fruit extract of Ananas sativa (MEAS) and its fractions, for anti-ageing bioactivity in the fruit fly, Drosophila melanogaster w1118 wild type flies.
Materials and Methods: Flies reared on food supplemented with 5, 10, and 20mg/ml fruit pulp and juice extract were assayed for longevity, fertility and stress resistance according to established protocols. Phytochemical composition and scavenging activity of extract on 2,2-diphenyl-1-picrylhydrazyl free radical (DPPH) were equally evaluated. The crude extract anti-ageing activity was assayed through a successive bioassay-guided fractionation approach, with different extraction solvents; n-Hexane, Chloroform, Ethyl acetate and Acetone.
Results: There was a dose-dependent effect of the fruit extract on lifespan, fertility and oxidative stress resistance, with a unique information on the anti-ageing bioactivity of Ananas sativa. The IC50 values of the fruit extract and Ascorbic acid positive control in the free radical scavenging activity were 248.15μg/ml and 81.51μg/ml respectively. However, the bioactive fraction obtained from the Ethylacetate fraction F1, did not exhibit oxidative stress resistant effect in the model organism.
Conclusion: The methanol fruit extract of Ananas sativa possesses anti-ageing bioactivity through oxidative stress resistance.
Keywords: Longevity; Ananas sativa fractions; antioxidants; free radical scavenging; oxidative stress resistance; Drosophila melanogaster.
