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African Journal of Traditional, Complementary and Alternative Medicines

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Apoptosis Inducing Effect Of Andrographolide On TF-47 Human Breast Cancer Cell Line

S Harjotaruno, A Widyawaruyanti, Sismindari Sismindari, NC Zaini

Abstract




Andrographolide isolated from Andrographis paniculata Ness (Acanthaceae) at 0.35 mM , 0.70 mM and 1.40 mM induced DNA fragmentation and increased the percentage of apoptotic cells when TD-47 human breast cancer cell line was treated for 24 , 48 and 72 h. The results demonstrated that andrographolide can induce apoptosis in TD-47 human breast cancer cell line in a time and concentration-dependent manner by increase expression of p53, bax , caspase-3 and decrease expression of bcl-2 determined by immunohistochemical analysis.

Keywords: Andrographis paniculata Ness , andrographolide, TD-47 cell , apoptosis.

African Journal of Traditional and Complementary Medicine Vol. 4 (3) 2007: pp. 345-351



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