Study on optimisation of extraction process of Tanshinone IIA and its mechanism of induction of gastric cancer SGC7901 cellapoptosis

  • J Hou
  • J He
  • X Jin
  • T Hu
  • Y Zhang
Keywords: Tanshinone ‡UA, MTT assay, gastric cancer SGC7901 cells

Abstract

The objective of this paper was to investigate the extraction process of tanshinone ‡UA and its mechanism of induction of gastric cancer SGC7901 cell apoptosis. Extraction process of tanshinone ‡UA was optimised by orthogonal experimental method, and its effect on gastric cancer SGC7901 cell apoptosis was observed using MTT assay and electron microscopy. The optimum extraction process of tanshinone ‡UA was as follows: addition of a 10-fold amount of 80% ethanol, one-time extraction, and extraction time of 45 minutes. The study concluded that tanshinone ‡UA can induce apoptosis of gastric cancer SGC7901 cells.

Keywords: Tanshinone ‡UA, MTT assay, gastric cancer SGC7901 cells

Published
2013-10-14
Section
Articles

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eISSN: 0189-6016