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Laboratory evaluation of the toxicity of methanolic extract of African bush tea seed (<i>Hyptis suaveolens</i> poit.) of the cowpea beetle (<i>Callosobruchus maculates</i> fabricius).


AK Musa

Abstract



Powdered seeds of Hyptis suaveolens Poit. (Labiatae) were extracted with methanol at 64-650 C in Soxhlet extraction unit. The extract was placed over a water bath at 600C to evaporate the methanol which was used as solvent. Newly emerged adults of Callosobruchus maculates F. (about 2-day old) were used in the evaluation of the toxicity of this extract. Different concentrations obtained by diluting the extract with acetone were prepared for use. The experiment was conducted in the laboratory at 25 + 20 C and 70 + 5% relative humidity. At all concentrations the extract had significant effect (P < 0.05) on C. maculates compared to the extract-free control (acetone alone).

Keywords: Callosobruchus maculates, evaluation, Hyptis suaveolens, methanolic extract.

Agro-Science Vol. 7 (2) 2008: pp. 114-117

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eISSN: 1119-7455