Antibacterial activity of extracts of Ocimum gratissimum on bacteria associated with diarrhoea
The antibacterial potential of cold water, hot water (100oC), ethanol and chloroform extracts of Ocimum gratisssimum leaves were tested against some pathogenic bacteria known to cause diaorrhoea; Staphylococcus aureus, Escherichia coli, Shigella sp. and Salmonella sp. Cold water and chloroform extracts did not show any effect on the test organisms. Only ethanol extract and hot water (100oC) extracts had inhibitory effects on the test organisms. All the test organisms were susceptible to ethanol extract with zones of inhibition which ranged from 6mm - 11mm and
minimum inhibitory concentration ranging from 30 - 60mg/ml. The test organisms were also susceptible to hot water extract with zones of inhibition ranging from 5mm - 10mm and minimum inhibitory concentration of range 25 - 70mg/ml. Due to the antimicrobial activity of Ocimum gratisssimum, it is therefore conceivable that it should be used to treat cases of diarrhea caused by the test organisms.
Keywords: Staphylococcus aureus, Antimicrobial activity, extracts, Diarrhoea, Bacteria