DETERMINATION OF SERUM ALBUMIN WITH TRIBROMOARSENAZO BY SPECTROPHOTOMETRY

  • Qing-Zhou Zhai
  • Jing-Mei Li
  • Jun-Pin Wang Research Center for Nanotechnology, Changchun University of Science and Technology, Changchun, 130022, P.R. China
Keywords: Serum albumin, Tribromoarsenazo, Emulgent OP, Human serums, Spectrophotometry

Abstract

The reaction of tribromoarsenazo(TB-ASA) with serum albumin in the presence of emulgent OP was studied by spectrophotometry. In a Britton-Robinson buffer solution at pH 2.9, tribromoarsenazo and bovine serum albumin can immediately form a red compound in the presence of emulgent OP with a maximum absorption wavelength at 354 nm. The presence of emulgent OP can increase the reaction sensitivity and the compound stability. The molar absorptivity of the compound is ε354 nm = 6.13 x 105 M-1•cm-1. Beer's law is obeyed over the range of 5.0-75.0 mg•L-1 for bovine serum albumin. The present method was applied to the determination of the total proteins in human serums with satisfactory results.
KEY WORDS: Serum albumin, Tribromoarsenazo, Emulgent OP, Human serums, Spectrophotometry

Bull. Chem. Soc. Ethiop. 2007, 21(2), 291-296.
Published
2007-07-10
Section
Articles

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eISSN: 1726-801X
print ISSN: 1011-3924