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Bulletin of the Chemical Society of Ethiopia

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DETERMINATION OF IRON IN CHINESE HERBAL MEDICINE BASED ON THE FLUORESCENCE QUENCHING OF RHODAMINE 6G

Yilin Wang, Jianping Lu, Hong Yang

Abstract


A fluorescence quenching method was developed for determination of Fe3+ with rhodamine 6G as fluorescent reagent. Fe3+ reacting with KI in diluted sulfuric acid produced I3- anion which reacted subsequently with rhodamine 6G to form a non-fluorescence compound. As a result, the compound quenched the fluorescence intensity of the system. The fluorescence intensity decreased linearly with the Fe3+ concentration in the range from 20 to 200 μg/L. The detection limit was 8.3 μg/L. This method has been applied to determine the iron in Chinese herbal medicine with satisfactory results.
KEY WORDS: Rhodamine 6G, Fluorescence quenching, Iron


Bull. Chem. Soc. Ethiop. 2009, 23(1), 113-116.



http://dx.doi.org/10.4314/bcse.v23i1.21307
AJOL African Journals Online