Isolation of chlorophyll a from spinach leaves

  • E.D. Dikio Department of Chemistry, North-West University, Private Bag X2046, Mmabatho 2735, South Africa
  • D.A. Isabirye Department of Chemistry, North-West University, Private Bag X2046, Mmabatho 2735, South Africa
Keywords: Chlorophyll a, Spinach leaves, Separation, Column chromatography, Solvent extraction, Pheophytin a

Abstract

An efficient method for separating chlorophyll a from spinach leaves by column chromatography and solvent extraction techniques has been developed. The purity and identity of the chlorophyll a have been confirmed by UV-Vis, IR and mass spectrometry. Yields from 100 g of freeze-dried spinach were 23 – 24 mg of chlorophyll a.

 

KEY WORDS: Chlorophyll a, Spinach leaves, Separation, Column chromatography, Solvent extraction, Pheophytin a

 

 

Bull. Chem. Soc. Ethiop. 2008, 22(2), 301-304.

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Articles

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eISSN: 1726-801X
print ISSN: 1011-3924