Hybridization of Palm Wine Yeasts ( Saccharomyces Cerevisiae) with Brewers Yeast by Protoplast Fusion
Keywords:
Protoplast fusion, palm wine Saccharomyces, ethanol
Abstract
Haploid auxotrophic strains of Saccharomyces cerevisiae were selected from palm wine and propagated by protoplast fusion with Brewers yeast. Fusion resulted in an increase in both ethanol production and tolerance against exogenous ethanol. Mean fusion frequencies obtained for a mating types ranged between 8 x 10-6 to 3.0 x 10-5. Intraspecific fusion between yeast strains gave a higher fusion frequency than interspecific fusion. High efficiency fusion was obtained between a mating types with fusion frequency ranging between 1.4 x 10-6 to 8.6 x 10-4. The fusion hybrids were uninucleated and stable showing actual karyogamy and prototrophy within six months of storage. Percentage regeneration of protoplast to viable, vegetative cells was between 52% to 77%. Minimal back mutations (reversions) were observed.Bio-Research Vol.1(1) 2003: 53-62 Key words: Protoplast fusion, palm wine Saccharomyces, ethanol
Published
2004-04-27
Issue
Section
Articles
Copyright: Creative Commons Attribution CC.
This license lets others distribute, remix, tweak, and build upon your work, even commercially, as long as they credit you for the original creation. This is the most accommodating of licenses offered. Recommended for maximum dissemination and use of licensed materials. View License Deed | View Legal Code Authors can also self-archive their manuscripts immediately and enable public access from their institution's repository. This is the final corrected version that has been accepted for publication and which typically includes author-incorporated changes suggested during submission, peer review and in editor-author communications.
