Preliminary studies on the endo-xylanase and acetyl esterase of Clostridium thermohydrosulfuricum (JW 102)

  • IM Ezeonu Department of Microbiology, University of Nigeria, Nsukka, Nigeria
  • AN Moneke Department of Microbiology, University of Nigeria, Nsukka, Nigeria
  • SKC Obi Department of Microbiology, University of Nigeria, Nsukka, Nigeria
Keywords: Hemicellulases, Clostridium thermohydrosulfuricum, xylanase, acetyl esterase, beeechwood xylan

Abstract

Clostridium thermohydrosulfuricum (JW102) produced low levels of xylanase in mineral medium containing yeast extract and beechwood xylan. Production of xylanase was highest at growth temperatures of 62 – 64oC. Both xylan and xylose supported acetyl esterase production. Xylose, as sole carbon source, supported the production of slightly more acetyl esterase than xylan, while enzyme produced in the presence of xylan was relatively more stable in the fermentation medium, maintaining over 80% of peak activity after 20 hours. Acetyl esterase activity was highest at assay pH of 6.5. At pH 5.5 and 6.0, enzyme activity was, respectively, 5% and 22% of that recorded at pH 6.5.

Key Words: Hemicellulases, Clostridium thermohydrosulfuricum, xylanase, acetyl esterase, beeechwood xylan.

Bio-Research Vol.1(2) 2003: 1-9
Published
2004-04-28
Section
Articles

Journal Identifiers


eISSN: 2705-3822
print ISSN: 1596-7409