Growth and α-amylase production by strains of Lactobacillus plantarum and Rhizopus oryzae cultures in cassava starch medium

  • Francois-Xavier Etoa Department of Biochemistry University of Yaoundé I, Cameroon
  • Roger D Djoulde Institute of Agricultural Research for Development of Cameroun, Maroua, P.O Box: 33 Maroua, Cameroon
  • Jean-Justin Essia Ngang Department of Biochemistry University of Yaoundé I, Cameroon

Abstract

A cassava starch medium was used to analyse the dynamics of batch growth and α-amylase production of strains of Lactobacillus plantarum and Rhizopus oryzae isolated from cassava dried chips. The strains displayed a growth of 0.5h-1 and 0.55 h-1, a biomass yield on cassava starch of 0.49g/g and 0.5g/g, a maximum specific cassava starch uptake rate (μm/Yx/s) of 2.98 mmol g-1 h-1 and 3.21 mmol g-1 h-1, a maximum oxygen uptake rate of 14.7 mmol g,sup>-1h-1 and 12.94 mmol g-1h-1, an oxygen efficiency of growth of 0.565 and 0.58, a carbon recovery of 1.035 and 0.97, and an energy recovery of 1.0 and 0.95 respectively. Enough α-amylase enzymes were synthesized, and cassava starch hydrolysis was not a limiting factor for growth of both strains. Theses results suggest the possibility to use these strains for developing a dual starter culture for cassava fermentation, and α-amylase production from cassava starch medium.

Keywords: cassava, α-amylase, Lactobacillus plantarum, Rhizopus oryzae, starter culture

Cameroon Journal of Experimental Biology Vol. 1(2) 2005: 107-115
Published
2006-08-31
Section
Full Articles

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eISSN: 1816-0573