Inhibition and Kinetic Studies of Tortoise (Kinixys erosa) Liver arginase
The effect of amino acid on tortoise liver arginase showed that L-lysine, L-valine, L-serine, L-aspartic acid and L aspartic acid had significant inhibitory effect on the enzyme but proline and glutamic acid showed slight inhibition. Ethylenediaminetetraacetic acid (EDTA), citrate, ascorbic acid, boric acid and sodium borate completely inactivated the tortoise liver arginase. Inhibition studies on the enzyme with a number of cations showed decreased arginase activity. While Mn2+ satisfies the metal ion requirement of tortoise arginase, Sn2+, Hg2+, Ba2+ and Co2+, to a lesser extent inhibited the enzyme. The enzyme was markedly sensitive to inhibition by Zn2+, Ni2+ and Mg2+. The enzyme was also completely inactivated by thiol compounds: reduced glutathione (GSH), cysteine and 2-mercaptoethanol.