Antibacterial Activity, Phytochemical and Proximate Analysis of Moringa Oleifera Seeds Against Clinical Isolates

Abstract

This study was carried out to determine the antibacterial activity, phytochemical and proximate analysis of Moringa oleifera seeds extracts against some clinical isolates. The antibacterial activity for Aqueous and Ethanol extracts was determined using the agar diffusion method. The Phytochemical Screening of the seed was carried out using Qualitative Analysis (QA) and proximate analysis was carried out according to AOAC methods. The aqueous seed extract showed varying degrees of antimicrobial activity over that of ethanol extract on the microorganisms tested. Two species (Staphylococcus aureus and Salmonella typhi) presented the lowest MIC of 31.25 and 125mg/ml compared to MBC of 250mg/ml respectively. The phytochemical revealed the presence of Flavonoids, Tannins, Alkaloid, and Saponins. The Proximate Analysis revealed high concentration of Crude lipid (35.58 ± 0.42%) and lowest concentration of Moisture content (2.03 ± 0.01%). This study shows that moringa oleifera seeds can be as effective as synthetic medicine to combat pathogenic microorganisms if properly utilized.

Keywords:   Moringa oleifera seeds; Proximate analysis; Phytochemicals; Staphylococcus aureus