Isolation and detection of antibiotic resistant genes of Escherichia coli isolated from urine samples of patients attending some hospitals in Dutse metropolis, Jigawa State, Nigeria

Abstract

Gram-negative E. coli bacteria are frequently seen in the human digestive system. It is an important source of nosocomial infection, providing a substantial public health risk. Multidrug-resistant (MDR) strains of E.coli that are challenging to treat with current antibiotics have emerged as a result of the expansion of the QNRA, QNRB and QNRS genes. It is an important source of nosocomial infection, providing a substantial public health risk. Multidrug-resistant (MDR) strains of E.coli that are challenging to treat with current antibiotics have emerged as a result of the expansion of the QNRA, QNRB and QNRS genes. To better understand the risk of MDR E.coli infections,it crucial to evaluate the prevalence of the QNRA, QNRB and QNRS genes. This study investigates the antibiotics resistant genes of Escherichia coli isolated from urine samples of patients collected from Dutse metropolis. To better understand the risk of MDR E.coli infections, it is crucial to evaluate the prevalence of the QNRA, QNRB and QNRS genes. The study’s aim was to isolate and detect antibiotic resistant genes in E. coli from urine samples of patients attending some hospitals in Dutse metropolis. A sample size of 162 urine samples was collected from UTI patients admitted to the two hospitals. The samples were analyzed using the phenotypic screening methods. The urines samples were sub cultured on MacConkey agar to isolate the bacterium (E. coli). Series of biochemical tests were conducted to confirm the bacterium. 21(12.1%) E.coli was detected in all of the samples. Five (5) different antibiotics (ciprofloxacin, nalidixic acid, levofloxacin, norfloxacin and ofloxacin) were used to carry out the sensitivity test on the 21 positive E. coli isolates. Nalidixic acid appeared to be the most sensitive (95.2%) and majority of the isolates were resistant to ciprofloxacin (85.7%). Polymerase chain reaction (PCR) was carried out to find out the presence of quinolone resistant genes (QNRA, QNRB AND QNRS) in E. coli isolates. These resistant genes were detected in six (6) of the isolates, which exhibited the highest resistant profiles among the 21 positive isolates tested. Among the resistant genes, qnrA was found to be the most prevalent 5(83.3%), while qnrB were 3(50.0%) and qnrS 3(50.0%) respectively. Future research should expand on these findings, including broader geographical sampling and  KeywordsAdd additional information for your submission. Press 'enter' after each term. comprehensive analyses of resistance mechanisms.