Prevalence and phenotypic characteristic of Klebsiella pneumoniae Carbapenemase producing Klebsiella pneumoniae isolates from clinical samples in Nasarawa-West, Nigeria.

Abstract

Klebsiella pneumoniae carbapenemase (KPC) is a newly identified group of pathogens associated with healthcare setting. The study was aimed to determining the prevalence Klebsiella pneumoniae carbapenemase (KPC) producing Klebsiella pneumoniae at some hospitals in Nasarawa West Senatorial district, Nasarawa State A total of 250 clinical samples comprising of urine (60), wound swab (46) , blood (60), stool (60), and sputum (24) were collected and subjected to cultural isolation followed by biochemical identification of the isolated organisms among which 33 Klebsiella pneumoniae (13.2%) were identified. The modified Kirby Bauer disk method was used to determine the isolate’s antimicrobial susceptibility. Carbapenemase production was successfully verified using modified Hodge test.. Out of the 250 clinical samples collected, the prevalence of Klebsiella pneumoniae was found to be 13.2%, with 33 isolates identified. Modified Hodge test positivity was observed in 27.3% of the Klebsiella pneumoniae carbapenemase (KPC) producers (n=9/33). Carbapenem resistant Klebsiella pneumoniae is emerging in Nasarawa west senatorial district even though carbapenem use is low.