Single cell protein extraction from orange wastes using Aspergillus niger isolate no. 5 under solid state fermentation

  • Tesfaye Alemu
Keywords: Aspergillus niger isolate, Orange wastes, Protein enrichment, Standardization of protein, Solid state fermentation.

Abstract

An attempt was made to optimize protein extraction from sweet orange wastes (SOW) and orange pulp wastes (OPW) using Aspergillus niger isolate No. 5 by solid state fermentation. Maximum protein enrichment of 52.48% and 46.50% were made using the substrates of SOW and OPW, respectively, on the seventh day of incubation at 30°C. The single cell protein quantified from the fungus grown on malt extract broth enriched with SOW and OPW were 23.14% and 21.35%, respectively; whereas the protein extracted from the growth of the fungus on potato dextrose culture broth enriched with SOW and OPW were 23.60% and 21.48%, respectively. The highest production of intracellular protein (70.46%) was obtained from OPW in comparison with that of SOW in soluble protein as intracellular protein (65.31%). It was clearly indicated that the standardization and determination method of protein using SOW and OPW wastes by A. niger isolate No. 5 has produced the maximum protein enrichment under solid state fermentation.


Key words/phrases: Aspergillus niger isolate, Orange wastes, Protein enrichment, Standardization of protein, Solid state fermentation.

Published
2016-07-15
Section
Articles

Journal Identifiers


eISSN: 1819-8678