Antibacterial activity of extracts from Myrtus communis L. (Ades) and Dodoneae angustifolia L.F. (Kitkita) using bioautography method

  • Negero Gemeda
  • Kelbessa Urga
  • Messay Getachew
  • Kissi Muddie
  • Frehiwot Teka
  • Ashenif Tadele
  • Hirut Lemma
  • Mulugeta Guta

Abstract

The increasing prevalence of antibacterial drug resistant organisms in our globe and high prevalence of infectious diseases in developing countries has led to new efforts in the search of bioactive compounds from complex chemical composition of plant extracts. A bioautographic agar overlay assay using Staphylococcus aureous as the indicator organism for the detection of antimicrobial compounds from ten extracts of Myrtus communis L. and Dodoneae angustifolia L was analyzed. Hexane, dichloromethane, acetone, methanol and water solvents are used as extractant and ethyl acetate: methanol: water, chloroform: ethyl acetate: acetic acid and benzene: ethanol: ammonia solvent systems were used to separate the components from all the extract of Myrtus communis L. and Dodoneae angustifolia L. Our results indicated that the extracts of Myrtus communis L.f. and Dodoneae angustifolia L had bioactive constituents responsible for their antibacterial potentials. Water solvents extracted small number of antibacterial compounds from both plants, followed by hexane extractant; while dichloromethane, acetone and methanol extractant shared similarities in bioactive compounds on bioautograms, and extracted the highest number of antibacterial compounds with variety of polarities. Chloroform: ethyl acetate: acetic acid solvent system separated the largest number of biologically active components in all extractants. As a high number of antibacterially active compounds were found in M. communis and D. angustifolia extracts of dichloromethane, acetone and methanol, we recommend assay guided fractionation, isolation and dosage formulation of these antibacterial compounds from these plants for clinical applications.

Keywords/phrases: Antibacterial, Bioactive-compound, Bioautography, Dodonaea angustifolia, Myrtus communis

Ethiop. J. Biol. Sci., 10(1): 57-72, 2011

Author Biographies

Negero Gemeda
Biomedical and Clinical Research , Department of Drug Research, Ethiopian Health and Nutrition Research Institute, P. O. Box 1242, Addis Ababa, Ethiopia
Kelbessa Urga
Natural Product Research, Department of Drug Research, Ethiopian Health and Nutrition Research Institute, P. O. Box 1242, Addis Ababa, Ethiopia
Messay Getachew
Natural Product Research, Department of Drug Research, Ethiopian Health and Nutrition Research Institute, P. O. Box 1242, Addis Ababa, Ethiopia
Kissi Muddie
Natural Product Research, Department of Drug Research, Ethiopian Health and Nutrition Research Institute, P. O. Box 1242, Addis Ababa, Ethiopia
Frehiwot Teka
Natural Product Research, Department of Drug Research, Ethiopian Health and Nutrition Research Institute, P. O. Box 1242, Addis Ababa, Ethiopia
Ashenif Tadele
Drug Formulation Research, Department of Drug Research, Ethiopian Health and Nutrition Research Inistitute, P. O. Box 1242, Addis Ababa, Ethiopia
Hirut Lemma
Biomedical and Clinical Research , Department of Drug Research, Ethiopian Health and Nutrition Research Institute, P. O. Box 1242, Addis Ababa, Ethiopia
Mulugeta Guta
Natural Product Research, Department of Drug Research, Ethiopian Health and Nutrition Research Institute, P. O. Box 1242, Addis Ababa, Ethiopia
Published
2013-06-14
Section
Articles

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eISSN: 1819-8678