Sensitivity pattern of clinical isolates of <i>Candida albicans</i> from hiv/aids patients to combined physcia grisea extract and tioconazole

Abstract

This study was carried out to investigate the sensitivity pattern of clinical isolates of C. albicans from HIV/AIDS patients to combined P. grisea extract and tioconazole. Twenty isolates of C. albicans were obtained from high vaginal swab (HVS) from HIV/AIDS patients in Bishop Shanahan Hospital, Nsukka after their confirmatory test. The sensitivity pattern of these isolates to P. grisea, tioconazole, and P. grisea combined with tioconazole was carried out using agar diffusion technique. The result of the in vitro study showed that P. grisea extract decreases the MIC of tioconazole thereby increasing the efficacy of the agent. The test microorganism, C. albicans was highly sensitive to P. grisea combined with tioconazole but less sensitive to the P. grisea extract alone. Overall, the antimicrobial activity of both the single and combined agents was found to be concentration dependent. This shows that in the treatment of candidiasis in HIV/AIDS patients, P. grisea extract combined with tioconazole may be used.