Comparative evaluation of the efficacy of Pseudomonas putida in the bioremediation of diesel fuel contaminated derno-podzoluivisolic soil of different horizons
This study was carried out to compare the efficacy of Рseudomonas putida, contained in the biopreparation «Pseudomin» in the bioremediation of diesel fuel contaminated derno-podzoluivisolic soil of two different horizons. By analyzing the Total Petroleum Hydrocarbons (TPH) content using IR-spectrometry method under laboratory conditions, results of the research showed that the inoculation of Рseudomonas putida in to the diesel fuel contaminated derno-podzoluivisolic soil samples in only 12 weeks was able to reduce the diesel fuel content up to 99.2% in the humus-accumulative horizon; and up to 92.8% in the eluvial horizon. Comparative evaluation of the contaminated variants inoculated with Pseudomonas of both horizons revealed a significantly higher rate of diesel fuel degradation in the humus accumulative horizon of up to 8.7 times, compared to the eluvial horizon, which shows a slightly slower rate of degradation.
Keywords: Bioremediation, petroleum hydrocarbon, humus-accumulative horizon, eluvial horizon, dernopodzoluivisolic soil, Рseudomonas putida, biological preparation «Pseudomin»