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Global Journal of Pure and Applied Sciences

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ISOLATION OF 2-METHOXYETHANOL DEGRADING BACTERIAL ISOLATE Pseudomonas sp. STRAIN VB

Ekhaise Frederick Osaro

Abstract


Four bacterial isolates from four different environmental samples (agricultural soil, compost soil and sewage sludge- anaerobic and aerobic wastewaters) were screened for their ability to mineralize 2-methoxyethanol under aerobic conditions. Isolate from anaerobic sewage wastewater was most efficient with an average of 48.6mmol/day mineralisation capacity. Morphological, physiological, biochemical and molecular characterization of the isolate showed that the strain, designated VB is of the genus Pseudomonas. 16S rRNA sequence analysis showed that the organism is related to Pseudomonas putida at 99.9% and Pseudomonas plecoglossicida at 99.8% similarity level. It is related to members of the genus Pseudomomas belonging to the rRNA group 1 within the gamma (g)Proteobacteria. The G+C content 64.5±0.8mol% is within the range characteristic of the genus Pseudomonas.

Key Words: 2-methoxyethanol, environmental samples, degradation, 16S rRNA and Pseudomonas sp. strain VB.

Global Jnl Pure & Applied Sciences Vol.10(1) 2004: 47-53



http://dx.doi.org/10.4314/gjpas.v10i1.16357
AJOL African Journals Online