ANTIDIABETIC EFFECT OF EMILIA SONCHIFORA IN DITHIZONE DIABETIC RATS
AbstractThe antidiabetic effect of a crude extract of Emilia sanchifora leaves was studied in rats with dithizone induced diabetes in rat. Three different concentrations of the crude chloroform – methanol extract were given orally to both normal and diabetic rats. The percentage blood glucose reduction for ES1 was 33.3, 14.2, 45.9, 49.7, 32.9, 31.1 and 17.3% after 0, 6, 12, 18, 24, 30 and 36 hours of oral administration. Significant difference of (P<0.001) occurs when the hyperglycaemic level of ES1 was compared with each group showing a remarkable reduction in blood glucose. The difference was also significant at P<0.01 with normal group and after six hours of oral injection of the extract. This shows that dithizone was able to induce significant (P<0./01) increase in blood glucose level. The extract showed a concentration dependent effect on blood glucose reduction hence ES3 (1.2 mg/ml) extract significantly (P<0.001) reduced blood glucose than ES1 (0.4mg/ml) after 12, 24 and 30 hours of oral injection when compared to ES2 (0.8 mg/ml) extract, the reduction in blood glucose was significant (P<0.001) after 6, 12, 18, 24 and 36 hours of oral administration. Chlorpropamide (DA) (diabenese) a known antidiabetic drug had the same glucose reduction pattern with the extracts. The peak percentage glucose reduction for DA, ES1, ES2 and ES3 were 32.3 after 24hours, 49.7 after 18 hours, 37.5 after 12 hours and 61.1% after 18 hours respectively. ES3 had the highest percentage reduction though DA has longer time of action.
The CO group showed raised levels of blood glucose throughout the work and showed significant difference P<0.05 with ES1 after 6, 12, 24 30 and 36 hours of oral injection
KEY WORDS: Dithizone diabetes, Emilia sanchifora, Chlorpropamide and Diabetes mellitus
Global Jnl Pure & Applied Science Vol.10(1) 2004: 183-187