Fusion of two yeast strains for enhanced crude oil degradation
AbstractA hybrid strain S24 was developed from Saccharomyces cerevisiae Y5A and Saccharomyces carlsbergensis Y7B, using protoplast fusion technique. The yeast protoplasts were prepared using gut juice from Helix pomatia (Land Snail) as cell digesting enzyme. The ability of the wild and the hybrid strains to degrade medium and light crude oil was determined gravimetrically. The parental strains Y5A and Y7A caused 56.9 and 52.6% weight loss respectively of light crude, while the hybrid caused 72.4% weight loss which was 19.3% and 16.6% higher than Y5A and Y7B respectively. Strains Y5A and Y7B also caused 46.7 and 4.1% weight loss respectively of medium crude while S24 caused 63.3% weight loss, which was 19.2 and 15.3% higher than the Y5A and Y7B. The results indicate that hybrid strains are potential candidates for crude oil pollution abatement programmes in oil spilled sites.
KEY WORDS: Protoplast fusion, Saccharomyces, hybrid strains, Snail juice, Biodegradation.
Global Jnl Pure and Applied Sciences Vol.10(4) 2004: 491-496