Generation Of Multicopy Pichia Clones From Gap Vector

Abstract

The methylotrophic yeast Pichia pastoris was used as a host to generate multicopy clones using in-vitro multimerization and GAP vector approaches. The latter approach relied on the selection of spontaneously occurring multiple integrants based on zeocin resistance. Higher levels of heterologous protein could result using these approaches.

Key Words: In vitro multimerization, GAP vector, Zeocin, Pichia

Global Journal of Pure and Applied Sciences Vol.11(1) 2005: 13-15