Generation Of Multicopy Pichia Clones From Gap Vector
Keywords:
In vitro multimerization, GAP vector, Zeocin, Pichia
Abstract
The methylotrophic yeast Pichia pastoris was used as a host to generate multicopy clones using in-vitro multimerization and GAP vector approaches. The latter approach relied on the selection of spontaneously occurring multiple integrants based on zeocin resistance. Higher levels of heterologous protein could result using these approaches.Key Words: In vitro multimerization, GAP vector, Zeocin, Pichia
Global Journal of Pure and Applied Sciences Vol.11(1) 2005: 13-15
Published
2005-02-18
Issue
Section
Articles
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