Histological study on the staining potentials of Aqueous extract of Ceratonia Siliqua bark

  • EE Okpidu
  • AU Okon
  • GP Oyadonghan
  • LA Ogbodo
  • ECN Onyenekwe
Keywords: Ceratonia siliqua, Extract, Haematoxylin and Eosin, Counterstain, General tissue structure.

Abstract

This study was designed to determine the staining potentials of aqueous extract of Ceratonia Siliqua bark adapted for the first time as a counter stain in Haematoxylin and Eosin staining reaction. The staining solution was used to stain liver, lung and kidney sections. Phytochemical screening of the extract revealed the presence of tannin (hennatonic acid or Lawsone), while results of the staining reactions showed that Ceratonia siliqua at a low pH, has intense staining qualities, confirming further that the effectiveness of a stain is influenced by the pH of its environment.

Key words: Ceratonia siliqua, Extract, Haematoxylin and Eosin, Counterstain, General tissue structure.

Published
2014-10-01
Section
Articles

Journal Identifiers


eISSN: 2384-681X
print ISSN: 2315-5388