Main Article Content

Performance of selective media for <i>E. coli</i> O157, using non-sorbitol fermenting strains and physiological characters of shiga-toxin producing species isolated in Côte d'Ivoire


A Dadie
M Dje
K Brou
M Dosso

Abstract

Performance of three selective media, tmperature and pH effects on growth of E. coli O157:H7 isolated in Ivory Coast were investigated. Thirty-eight (38) non-sorbitol fermenting E. coli were characterized by serotyping, detection of shiga-toxin gene (PCR) and plating on SMAC, VRMUG and BCMtm media for Performance evaluation. Temperature and pH effects on E. coli O157:H7 were measured in varied culture conditions. All culture media were sensitive (100%) for the growth of E. coli O157. Their specificities were 97.3, 94.7 and 50.7% respectively for VRMUG, BCMtm and SMAC media. Predictive value for VR-MUG medium was 66%, whereas that for SMAC medium was only 5.4%. Serotype O157:H7 E. coli population grew slightly at 42 °C and didn’t grow under acid conditions (pH 4.5, 3.5). The performance of the culture media evaluated
and the physiological data obtained for E. coli O157:H7 during this study would contribute to improve both isolation and identification methods of the pathovars.

© 2010 International Formulae Group. All rights reserved.

Keywords: E. coli O157H7, performance, culture medium, shiga-toxin, pH, temperature


Journal Identifiers


eISSN: 1997-342X
print ISSN: 1991-8631