Wound healing biomolecules present in four proposed soft aqueous extractions of <i>Ageratum conyzoides</i> Linn

Abstract

Biochemically wound healing process is promoted by active agents which compose aqueous and alcohol herbal extracts and those components act synergistically. We propose in this study four methods of soft aqueous extraction (A, B, C, D) of Ageratum conyzoides Linn. leaves, a well-known west african plant for its wound healing properties. Our main objective was to know which of the extraction methods would allow us to obtain specifically tannins and flavonoids. We also investigated which types of flavonoids extracted could inhibit serine proteases like thrombin or serine proteases present in bacterial and then being bactericide. Ageratum conyzoides Linn. samples were collected in the beginning of the raining period in May 2019 in two regions of Togo. After aqueous extractions, TLC and HPLC-ESI+-QTOF analysis were done. Chromatographic fingerprints obtained were quite similar for the four extracts investigated. However, additional spots with different colors appeared for the B extract. Flavonoids were revealed by the numerous colors of spots observed on the chromatograms. Superposing HPLC-ESI+-QTOF chromatographic profiles of the four extracts, we deduced that our proposed extraction methods lead to same chemical compounds but B extract contained higher concentrations of later substances. Identified molecules belong to terpernoids and flavonoids classes.

Keywords: Ageratum Conyzoides Linn., Aqueous extractions, Biomolecules, Wound healing.