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Influence of sugars, pre-treatment with acid and suspending medium on the interaction of <i>Salmonella typhimurium</i> and <i>Salmonella enteritidis</i> with concanavalin A.
Abstract
The influence of experimental variables on the interactions of Salmonella Typhimurium L1388 (ST) and Salmonella Enteritidis L1225 (SE) with concanavalin A (con-A) were assessed. At a con-A concentration of 78.1 µg ml-1, ST was agglutinated and SE was not. The con-A-induced agglutination of ST was completely inhibited by equal concentration of D-(+)-mannose, D-(+)-glucose, D-(+)-galactose, N-acetyl-D-glucosamine, trehalose, β-D-(+)-cellobiose and raffinose. Pre-treatment of the bacterial cells with acid did not alter the con-A reactivity of ST and SE. Change in the suspending medium from PBS to immunogold buffer did not alter the con-A reactivity of ST and SE. Interactions of lectins with microorganisms have great diagnostic clinical laboratory value in microbial typing, taxonomic classification and epidemiology.
© 2007 International Formulae Group. All rights reserved.
International Journal of Biological & Chemical Sciences Vol. 1 (1) 2007: pp. 67-70
