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Determination of glyphosate by high performance liquid chromatography (HPLC) without prior extraction

AJ Yapo
A Dembele
AS Ello
A Trokourey


The aim of this study was to design a glyphosate analysis method. This molecule is an organic pollutant from water and soil. We have developed a chromatographic method with phenylisothiocyanate. This molecule
has allowed obtaining an intermediate molecule with the glyphosate being easily detectable in chromatography. The peak relating to this intermediate was identified using a comparison with several samples, including a
blank. The tests determined the retention time (RT) of glyphosate at 1.6 min and the values of the percentage of accuracy and repeatability of the method.