Determination of glyphosate by high performance liquid chromatography (HPLC) without prior extraction

  • MIG Bi
  • AJ Yapo
  • A Dembele
  • AS Ello
  • A Trokourey


The aim of this study was to design a glyphosate analysis method. This molecule is an organic pollutant from water and soil. We have developed a chromatographic method with phenylisothiocyanate. This molecule
has allowed obtaining an intermediate molecule with the glyphosate being easily detectable in chromatography. The peak relating to this intermediate was identified using a comparison with several samples, including a
blank. The tests determined the retention time (RT) of glyphosate at 1.6 min and the values of the percentage of accuracy and repeatability of the method.

Journal Identifiers

eISSN: 1997-342X
print ISSN: 1991-8631