Analgesic and anti-inflammatory activities of ethanolic extract of rheumatic tea formula (RTF) in rats and mice
The analgesic and anti-inflammatory activities of the ethanolic extract of Rheumatic Tea Formula (RTF) a polyherbal tea consisting the leaves of Eucalyptus globulus, Albizia chevalieri and bark of Salix alba were studied in mice and rats using acetic acid induced writhing, hot plate method, formalin induced pain and carrageenan induced rat paw edema. The extract at doses of 50, 100 and 200 mg/kg produced a significant (P<0.05) and dose dependent inhibition of writhing induced by acetic acid. In the hot plate method the extract at all doses also produced a significant (p < 0.05) dose dependent increase in latency of pain. In formalin induced pain the extract exhibited significant (P<0.05) analgesic activities in both first and second phase of the formalin induced pain at doses of 250, 500 and 1000 mg/kg. The ethanolic extract significantly (P<0.05) and dose dependently reduced paw edema induced by carrageenan. The present study suggests that RTF contains bioactive constituents that possess analgesic and anti-inflammatory effects, the former being mediated centrally and peripherally justifying its ethnomedicinal use in pain and inflammation associated with rheumatism.
Key words: Rheumatic Tea Formula, Analgesic, Anti-inflammatory, Acetic –acid, Carrageenan