Isolation and characterization of campest-5-en-3-ol from the root bark of Uvaria Afzelii
This study aimed at the isolation and characterization of a steroidal compound from the root bark of Uvaria afzelii. The methanolic crude extract of the root bark was suspended in aquous methanol and partitioned with n-hexane to obtain a fraction rich in volatile oils. The n-hexane fraction was subjected to column and preparative thin layer chromatography for isolation and purification of compounds. The n-hexane fraction yielded one steroidal compound. The structure of which was determined by 1H-, 13C- and DEPT135-NMR as campest-5- en-3-ol. The spectroscopic data compared very well with published data.
Key words: Extract, Uvaria afzelii, chromatography, campest-5-en-3-ol.