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Comparative Modelling and Binding Site Prediction of Gp 15 / 400 Polyprotein of Wuchereria bancrofti by Using Computational Approaches

M Mishra, AB Pant, P Srivastava

Abstract


Lymphatic filariasis is a tropical disease caused by filarial parasites Wuchereria bancrofti and Brugia malayi that are transmitted to human beings by mosquitoes. It is evident from previous researches that GP 15 / 400 polyprotein is an important protein found in Wuchereria bancrofti which can be used as good target for filarial disease as it is only found in this filarial nematode, but not in human beings. GP 15 / 400 polyprotein
is highly conserved and has multifarious roles in the survival and pathogenesis of this parasite, therefore, plays a significant role in disease development. Protein three dimensional structures are functionally very important and play an important part in progression of the disease hence these 3D structures are better target for further drug designing and relative studies. In the current paper we have developed 3D model structure of GP 15 / 400 polyprotein of Wuchereria bancrofti of filarial nematode. The  resulting model consisted of 90.5% of the residue falling in the
most favoured regions. Since functional site prediction is an integral step, therefore, in the current analysis, we predicted putative functional site residues as 227 ASP 28, 234 TYR 29, 261 GLU 31, 654 ASP 83, 679 LEU 86, 687 GLY 87, 701 ILE 98, 709 THR 90, 782 HIS 99, 817 PRO 103 in the GP 15 / 400 polyprotein also. Molecular model of this protein of (filarial nematode) as documented in this study may provide a valuable aid for designing an inhibitor or better ligand against filarial disease which could play a significant role in the future drug designing of this serious
disease.

Keywords:  Filariasis, Functional sites, GP 15 / 400 polyprotein, Homology Modeling, Wuchereria bancrofti.




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