Article Sidebar
Published:
Feb 11, 2015Keywords:
Article Details
Open access articles published in the Journal of Agriculture, Science and Technology are under the terms of the Creative Commons Attribution (CC BY) License which permits use, distribution and reproduction in any medium, provided the original work is properly cited. The CC BY license permits commercial and non-commercial re-use of an open-access article, as long as the author is properly attributed.
Copyright on any research article published in the Journal of Agriculture, Science and Technology is retained by the author(s). The authors grant the Journal of Agriculture, Science and Technology with a license to publish the article and identify itself as the original publisher. Authors also grant any third party the right to use the article freely as long as its original authors, citation details and publisher are identified.
Use of the article in whole or in part in any medium requires proper citation as follows:
Title of Article, Names of the Author, Year of Publication, Journal Title, Volume (Issue) and page. Links to the final article on the JSRE website are encouraged.
The Creative Commons Attribution License does not affect any other rights held by authors or third parties in the article, including without limitation the rights of privacy and publicity. Use of the article must not assert or imply, whether implicitly or explicitly, any connection with, endorsement or sponsorship of such use by the author, publisher or any other party associated with the article.
For any reuse or distribution, users must include the copyright notice and make clear to others that the article is made available under a Creative Commons Attribution license, linking to the relevant Creative Commons web page. Users may impose no restrictions on the use of the article other than those imposed by the Creative Commons Attribution license.
To the fullest extent permitted by applicable law, the article is made available as is and without representation or warranties of any kind whether express, implied, statutory or otherwise and including, without limitation, warranties of title, merchantability, fitness for a particular purpose, non-infringement, absence of defects, accuracy, or the presence or absence of errors.
Main Article Content
Identification of <i>Glossina morsitans morsitans</i> odorant binding proteins genes in <i>Glossina fuscipes fuscipes</i>: a preliminary study
Abstract
Tsetse flies are vectors of trypanosome parasites, causative agents of Trypanosomiasis in humans and animals. Odorant Binding Proteins (OBPs) are critical in insect olfaction as they bind volatile odours from the environment and transport them to receptors within olfactory receptor neurons for processing providing critical information for host identification. Glossina morsitans morsitans primers designed for conventional PCR were used to screen OBP genes in Glossina fuscipes fuscipes in female head, thorax, abdomen and leg tissues. A total of 31.8% of OBPs were identified in G. f. fuscipes head while 18.2% were detected in the thorax. The abdomen had 22.7% OBPs identified and the legs revealed 18.2% OBPs. OBP5 and OBP6 were the most predominant since they were detected in the head, thorax and abdomen of G. fuscipes fuscipes which may provide the need to identify their functions in both G. morsitans morsitans and G. fuscipes fuscipes. This study confirms genetic differences between OBPs from riverine (G. f. fuscipes) and savanna (G. m morsitans) species which may be key in understanding the role of olfaction in enhancing vector competence of G. m. morsitans and G. f. fuscipes. Such information will be critical in designing better vector control strategies based on olfactory mediated behavior.
Key words: Trypanosomiasis, olfaction, tsetse flies, odorant binding proteins
