Isolation of lupeol from the stem bark of <i>Leptadenia hastata</i> (Pers.) Decne

Abstract

Dried stem bark powder of Leptadania hastata was subjected to maceration with methanol to afford crude methanol extract, which was partitioned with n-hexane, ethylacetate, chloroform and n- butanol to afford different their respective fractions. Extensive phytochemical screening of the n-hexane fraction using column chromatography resulted to the isolation of a white solid substance. The substance was identified as of lupeol using IR, 1D – NMR, 2D – NMR data and by comparison with reference spectral data.

Keywords: Leptadenia hastata, Isolation, Lupeol, 1D – NMR, 2D – NMR