Chromosomes Behavior at Meiosis in <i>Chlorophytum stenopetalum</i> Bak at Pachytene, Diakinesis and Diplotene

Abstract

Meiotic prophase is classically subdivided into five stages: leptotene,  zygotene, pachytene, diplotene, and diakinesis. The objective of this paper is to evaluate the behaviours of Chromosomes in Chlorophytum stenopetalum Bak.at pachytene /diakinesis and metaphase. The flower buds at right age  were harvested, fixed in Cornoy’s solution (3 part of absolute alcohol and 1 part of acetic alcohol) and preserved in a refrigerator at -4°? for at least thirty minutes. The flower buds were then hydrolyzed in 10% HCl for 3-5 minutes. Prepared slides were viewed using an Armscope microscope equipped with digital automatic camera. At diakinesis seven bivalents (7 II) were   predominantly observed (74.2%). Chromosomal stickiness is quite evidence. In addition, cross configuration and its resultant, ring formation at metaphase indicate the presence of translocation heterozgosity in the chromosomes of the species investigated. These abnormalities are likely to affect  microsporogenesis and pollen viability.