Evaluation of antioxidant and antimicrobial activity of Spergularia marina (L.) Griseb extract
Spergularia marina (L.) Griseb is a species from spergularia genus (Caryophyllaceae). This genus has some medicinal effects such as antidiabetic, antioxidant and diuretic effect. For many centuries people have been trying to alleviate and treat diseases with natural resources. Free radicals and oxidative agents cause disorders such as cardiovascular diseases, pulmonary diseases and rheumatoid arthritis. Synthetic antioxidants have toxic effects, so scientists are trying to find new antioxidants from natural resources such as plants. Nowadays plants are known as an important resource for treating different diseases such as: common cold, cough, diarrhea, bronchiolitis, respiratory infections, UTI, and skin lesions, because of this reasons, a lot of plants have been studied to make new drugs. Due to increasing drug resistant in microorganisms, it is necessary to find new compounds with antimicrobial properties. In this work, firstly the total phenolic and flavonoid contents of Methanolic extract and Chloroform, Ethyl acetate and Water fractions from Spergularia marina (L.) Griseb were calculated, and then the antioxidant activites of all samples were determined using three methods, DPPH radical scavenging, Reducing power and Ferrous Chelating activity assay. In this assays VitC, VitE, BHA, EDTA, rutin and gallic acid were used as reference compounds. The antimicrobial activities of Methanolic extract from all part of plant determined against standard strains include: Staphylococcus aureus, Escherchia coli, Bacillus subtilis, Pseudomonas aeruginosa, Streptococcus pneumoniae, Salmonella enterica, Klebsiella pneumoniae, Serratia Marssns and Candida albicans by disc diffusion method. In all antioxidant methods, Methanolic extract was showed the highest antioxidant activity and chloroform extract was the lowest antioxidant activity (aerial parts, seeds and all parts of plant). The results show methanolic extract of all parts of plant have any effect against above microorganisms.
KeyWords: Spergularia marina; caryophyllaceae; antioxidant; antimicrobial; DPPH;
reducing power; ferrous chelating activit.