Journal of Fundamental and Applied Sciences <p><span lang="EN-US">Journal of Fundamental and Applied Sciences is an international journal reporting significant new results in all aspects of fundamental and applied sciences research. We welcome experimental, computational (including simulation and modelling) and theoretical studies of fundamental and applied sciences. The work must fall into one of the four sections of the journal. If the work overlaps two or more journal sections then it can be submitted as an interdisciplinary fundamental or applied sciences paper.&nbsp;</span></p> <p><span lang="EN-US">You can view this journal's website <a href="" target="_blank" rel="noopener">here</a>.&nbsp;</span></p> University of El Oued en-US Journal of Fundamental and Applied Sciences 1112-9867 The copyright belongs to the journal. A new technique for sweat chloride determination using Mohr’s silver method: Development and analytical validation <p>This study presents a new technique for determining chloride ions in the sweat test for the diagnosis of cystic fibrosis. The technique developed is based on Mohr’s silver titrimetric method. The analytical development was performed on three quality control levels (C1, C2 and C3 at 25, 45 and 65 mmol/L respectively) and validated according to the requirements of ISO15189. Repeatability was estimated by the coefficient of variation (CV) at 2.45%, 3.12% and 2.21% for C1, C2 and C3. The CV estimated the intermediate precision at 4.12%, 4.51% and 2.63% for C1, C2, C3. The bias estimated the accuracy at 0.99% for C1, 2.76% for C2 and 2.55% for C3. The assays were performed following a linear calibration curve (equation: Y=0.0001 X + (3 x 10-5) with R<sup>2</sup> =0.9995) between 0 and 150 mmol/L. This new technique is a simple and fast alternative using stable and less toxic reagents than the mercurimetric technique.</p> Nardjess Rim Laoufi Farida Bouchenak Amina Zouakh Mouna Abdelaziz Abdelhakim Zerrouk Djamal Saddouki Khaled Sobhi Copyright (c) 0 2023-04-12 2023-04-12 15 2 127 137 10.4314/jfas.v15i2. Response of okra cultivars <i>Abelmoschus esculantus</i> (l.) Meonch to the different levels of nitrogen and di-ammonium phosphate <p>An experiment conducted to know the effect of nitrogen and DAP on growth and yield of okra cultivars. It laid down in ( SRCD), with three replications. The treatments are control Co , two levels of nitrogen and DAP referred to as; N 1 , N 2 , D 1 , and D 2 , it is equal to 0, 36, 72, 72, 144 g plot<sup>-1</sup> , respectively, and cultivars of Bassnty, Jamoia, Khartomia . The results revealed that treatments significantly affected all parameters except yield per plant and hectare. Higher nitrogen gave the heaviest fresh and dry weight, while control gave higher dry matter. The greatest number of pods per plant recorded by the higher dose of DAP. Bassanty significantly increased vegetative and productive parameters and slightly pod yield per plant. Bassanty received the higher dose of DAP significantly increased g rowth, while Jamoia with lower dose of nitrogen increased yield components. This result concludes th at the att ributes of okra influenced by both treatments.</p> Abdalla Mohamed Hussein Copyright (c) 0 2023-04-12 2023-04-12 15 2 138 146 10.4314/jfas.v15i2. Assessment of some heavy metals and physicochemical parameters in hand-dug well and borehole water sample from Mubi South L.G.A Adamawa State <p>An attempt has been made to detriment the concentration level of some heavy metals and physicochemical parameters in hand dug Well and Borehole water of Mubi local government area of Adamawa state with the aid of AAS , pH and conductivity meter. Water samples were collected from ten sites which serves as drinking water sources and subjected to analysis for determination of Pb , Cd and Cr. The results hows equal concentration of Pb 0.048±0.00 mg//L) detected from three samples, the concentration level of Cd and Cr ranges between 0.088 to 0.439 mg/L and 0.250±0.165 to 0.750±0.333 mg/L respectively, this indicate that all the three heavy metals detected were above the standard limit set by WHO and SON for drinking water. pH, conductivity and TDS values ranges between 6.94 to 8.14, 362 to 1063 µS/cm and 199.10 to 584.65 mg/L respectively, these were less than the value set by WHO.</p> Muhammad Abubakar Ibrahim Tajo Sirajo Copyright (c) 0 2023-04-12 2023-04-12 15 2 147 159 10.4314/jfas.v15i2. Safe use of fluoropyrimidines in oncology: Development of a method for the determination of plasma uracil and dihydrouracil levels by UHPLC-MS-MS and screening for Dihydropyrimidine Dehydrogenase deficiency <p>5-Fluorouracil (5-FU) is a widely used drug in oncology. It can cause toxicity, especially in the case of Dihydropyrimidine Dehydrogenase (DPD) deficiency. An enzyme responsible for the inactivation of more than 80% of the 5-FU dose and for the transformation of uracil (U) into dihydrouracile (UH2). A sensitive method is needed to screen for DPD deficiency by determination of U level, as recommended by the European Medicine Agency (EMA). Separation was performed by an UHPLC on a C18 column and a tandem mass spectrometer performed the detection of U and UH2. The method’s performance was validated according to ICH M10 recommendations. The validated method was used to screen for DPD deficiency in fifty-seven hospitalized patients. None of the patients showed DPD deficiency based on the U-rate instead of the metabolic ratio UH2/U (7.08%) which might be a more sensitive tool for this screening</p> Farida Bouchenak Nardjess Rim Laoufi Khaled Sobhi Djamal Sadouki Mohamed Hazi Samir Adjmi Kamel Mansouri Kamel Bouzid Copyright (c) 0 2023-04-12 2023-04-12 15 2 160 175 10.4314/jfas.v15i2. Mild steel corrosion inhibition in acidic media using <i>Sarcocephalus latifolius</i> leaves extract <p>At temperatures between 303K and 323K, three different concentrations of the methanol extract from <em>Sarcocephalus latifolius</em> were utilized to inhibit the corrosion of mild steel coupons in a 0.9M HCl solution. The plant extract adhered to the surface of mild steel metal under the investigated conditions, according to surface characterization techniques including SEM and FT-IR. It has been proven through weight loss trials in this work that the plant extract's ability to suppress growth improves with both an increase in its concentration and a decrease in temperature. The corrosion and inhibition processes were presented as being viable and spontaneous using thermodynamic parameters. Additionally, it was determined that the inhibitory process followed first order kinetics, with half life values rising as plant extract concentration did. Calculated activation energy values and isotherm characteristics demonstrated that the process adheres to the physical adsorption mechanism.</p> Fater Iorhuna Abdullahi Muhammad Ayuba Copyright (c) 0 2023-04-12 2023-04-12 15 2 176 193 10.4314/jfas.v15i2.