Main Article Content

A Comparative Study of the Effect of Hydrogen Peroxide and Polyhexamethylene Biguanide Contact Lens Solutions on Pseudomonas aeruginosa, in vitro.


OU Amaechi
CA Ohadugba

Abstract

Contact lens cases contaminated with Pseudomonas aeruginosa are a major risk factor in ocular infections. A comparative study of the effect of 0.6% hydrogen peroxide and 0.0005% polyhexamethlylene biguanide on Pseudomonas aerugunosa isolated from three different sources, and cultured on nutrient agar plates and one drop of the undiluted disinfectant. Result showed that the 0.6% hydrogen peroxide had a wider diameter of zone of inhibition (466+1.00mm)than polyhexamethylene biguanide (335 + 5.00mm) indicating better disinfectant activity. This difference was significant using the Z-test at 0.05 level of significance (p>0.05). Furthermore, the diameters of the zones of inhibition decreased with serial dilution of the disinfectants. The minimum inhibitory concentration of hydrogen peroxide was 6x107 while that of polyhexamethylene biguanide was 5x10 -8 or 12 times a more effective disinfectant. Hydrogen peroxide is a very effective contact lens disinfectant solution in order to avoid possible pseudomonas infection of the eye.

Key words: Contact lens, disinfectant Pseudomonas aeruginosa, ocular infection.


Journal Identifiers


eISSN:
print ISSN: